Parts Registry

From CSBLwiki

Problems

• ccdB is not working well...?!
  • source#1
  • source#2
  • source#3 PDF
    • LR Reaction: High background in the absence of the entry clone
      • LR reaction transformed into an E. coli strain containing the F' episome and the ccdA gene
      • Use an E. coli strain that does not contain the F' episome for transformation (e.g. DH5a™, TOP10)
• DH5a is not a good host for ccdB
• DH10B is o.k.
• SURE?
• BL21(DE3)?