Primer Verrucosispora gap

From CSBLwiki

Contents 1 Primer Naming Format 1.1 Gap primer format 2 List of primer 2.1 primers

Primer Naming Format

Gap primer format

• >[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse]

ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R
 
                     CONTIG 1                                                     CONTIG 2
                                ------>
         5'                           3'  
 --------CTCAGGTCAGGCAGGCGTGATGATCTCTTC----------------NNNNNNNNN----------------------------------------
                                                                        GTTAGAGGAGAGTGCGGCTGGGCTCCAATCAT
                                                                        3'                             5'
                                                                        <-------

Positions:                      1105bp                                      529bp

List of primer

• By Using Primer3
• "Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer).

primers

• 115 gaps (230 primers)

 >scaffold00001_2_3_751_F
 CAGAACTGGAGAACGTCCATGCTCGAGAAC
 >scaffold00001_2_3_1360_R
 TCGATCACCAGCTCACGGAAGTCCTCGTTG

 >scaffold00001_3_4_586_F
 GTCAGTGCCGATCAGGATGTTGTCCAC
 >scaffold00001_3_4_183_R
 ATCAACTGCCAGAACTCGTCGGTCCTCATG

 >scaffold00001_4_5_568_F
 TACGTCGAGGAACTGGGCGACTTCAAGACC
 >scaffold00001_4_5_838_R
 CATGTCGTCGATCTGCACGTTGACCAG

 >scaffold00001_9_10_1135_F
 AGACCGGATTGAGTGCTTCCTCTGCTGGTG
 >scaffold00001_9_10_1408_R
 CGACCAATTTCCGACGAGGTGACATTC

 >scaffold00001_11_12_354_F
 AAGGTCAGGATGGCGGTGAACACCTTGGTC
 >scaffold00001_11_12_345_R
 ATGTTGTCGCTGACCTTCGCGATACGGGAG

 >scaffold00001_12_13_1313_F
 TTTCAGGCTTTGAGGATCGAGGTAAACAGG
 >scaffold00001_12_13_315_R
 TCGCATCTTCTCAGCCAGTTCCGAG

 >scaffold00001_15_16_126613_F
 GTTCCCGGTTGCGATCGGTGAGGAT
 >scaffold00001_15_16_569_R
 CTTCTACCACGACACACACACCGATCC

 >scaffold00001_17_18_225_F
 GATCGTGACGAACGTGAAGGTCCAC
 >scaffold00001_17_18_408_R
 CAGATCCGGTACAGCTCCTTGTATTCCAG

 >scaffold00001_18_19_320_F
 CAGATAGATGGTGAAGTCAGGACGACGG
 >scaffold00001_18_19_177_R
 ATGAACTTTCCACCGATGCCGGTCGTCTTC

 >scaffold00001_21_22_243_F
 AAGGACGGACGTGGACATCGATCAGCAATC
 >scaffold00001_21_22_1379_R
 AGGACTCCTGTACGTCGATCACGACGAGTG

 >scaffold00001_23_24_410_F
 ACTGACCACGGTGCAGCTCTACTTCAC
 >scaffold00001_23_24_1184_R
 AGCGTGTAGTCGGCGGTATACACCTTC

 >scaffold00001_25_26_203_F
 GTCGAGGGGATCAACACAAGTGGAATCAAG
 >scaffold00001_25_26_184_R
 GTACCGTCGACAAGCAACTCCTGATCGTC

 >scaffold00001_27_28_280_F
 TGACGTGAGGGGACTACGGATGTAC
 >scaffold00001_27_28_260_R
 ACTTCGACAGGTAGGCCTTGCAGAG

 >scaffold00001_31_32_566_F
 AAACGAGCCGTACAACGGGCAACTG
 >scaffold00001_31_32_169_R
 ATCTTCCTGACCTCGTCGGTGTGGTACTTC

 >scaffold00001_34_35_1071_F
 CAGTACGTCATCTTCGACCGGACCATTTG
 >scaffold00001_34_35_2096_R
 AGTCGTCGTCGTCGAACATCGTGAC

 >scaffold00001_35_36_685_F
 GTCGTTCAGGTTCAGGTAGAACTCGGTGAC
 >scaffold00001_35_36_0_R
 CCCGACCGAACGGCACGCACACAA

 >scaffold00001_36_37_1073_F
 GATCGGTTTGCCGCGGTCGTTGTATTCGAC
 >scaffold00001_36_37_740_R
 ATACGGCTTCGTCGAGAACCTGACC

 >scaffold00001_38_39_612_F
 AACATGTCGTTGAGCGCGGCCAGTTTG
 >scaffold00001_38_39_672_R
 CTCTTCGACGAGATCATCCAGGTGCTG

 >scaffold00001_41_42_205_F
 ACCGAAGCAGTCGAAGACGGTACAC
 >scaffold00001_41_42_339_R
 GTCTTCTTCTGGCCCCGCAACGAGATCATG

 >scaffold00001_42_43_389_F
 TAAAATCTGTGATCTGGGCTAACCTTGGGG
 >scaffold00001_42_43_223_R
 GTGATCGGTGTGTTCGTGGCTGACCAGTAC

 >scaffold00001_45_46_401_F
 GGATCAGCTCCGGATAGTTGAAGTCGTC
 >scaffold00001_45_46_423_R
 ACAACGAGGACTTCGACAACGGCTTCAAGG

 >scaffold00001_47_48_1970_F
 GACGACTACCTGCGTAAACCCTTCGACCTG
 >scaffold00001_47_48_1044_R
 CACCAATCTGCTTGTGGGTCTGGTGCTGAC

 >scaffold00001_48_49_1639_F
 GATGTTCATCGACCTGGAGAACGTGC
 >scaffold00001_48_49_244_R
 CATCTCTCCCGTCAGCTCATCGTTCGAAG

 >scaffold00001_49_50_1297_F
 CAGGGTTTCCTGAGCCACCTTGAAC
 >scaffold00001_49_50_822_R
 CTGTTCTCCCAGGACCCGATCTTCAACTC

 >scaffold00001_50_51_227_F
 CGTGCCATGTTCTGAACGAGAACGATC
 >scaffold00001_50_51_860_R
 GTGACCAGCGAGTGAATCTTGTCGCGTTG

 >scaffold00001_55_56_239_F
 CACCTGACCTCTGACAATGAACGCCGAAAG
 >scaffold00001_55_56_206_R
 GATTGGCTTCTATACAAACTAAGGTGGCGC

 >scaffold00001_57_58_1204_F
 TCATGCTGTCCGTATCCATTTAGGACCTTC
 >scaffold00001_57_58_939_R
 TTCTACCGTGCCTACTTCGAGACCGAC

 >scaffold00001_61_62_285_F
 TCAGGTTGAGATCGACCATCGCGAG
 >scaffold00001_61_62_944_R
 TTGCACCATCAGCTCAGCATCGTCTTCGAC

 >scaffold00001_62_63_898_F
 CTGATGATCAAGGTGTTGCGGCTGTC
 >scaffold00001_62_63_367_R
 GTTGTAGCCGCCGAAGTCGTTCATGCTGAC

 >scaffold00001_67_68_5122_F
 GACCGTTGCGACCAGATATTCGGCGATT
 >scaffold00001_67_68_0_R
 AACCGCTGACCGCGTTCGTGCTGTTCT

 >scaffold00001_71_72_349_F
 AACCCACGCATCCCACTCAACGAGCTGAAC
 >scaffold00001_71_72_0_R
 TCGTTGTCCACCACGCCGACGAACA

 >scaffold00001_73_74_2968_F
 TACGCGGCAGTCGGACGGGTGTGTT
 >scaffold00001_73_74_0_R
 GCTCACCGTGCCGGTGAGGACCAA

 >scaffold00001_75_76_788_F
 GTCGACGTTTCATCACGTCACTGGGTG
 >scaffold00001_75_76_0_R
 GTGCGCCAAGACCTGCGCGAACAGT

 >scaffold00001_76_77_1854_F
 GTGATCAAGGTGATCGAAGCCCTGC
 >scaffold00001_76_77_535_R
 ATCTACAACCACCTGGGCGGCAAGGAGAAC

 >scaffold00001_80_81_426_F
 CACTGCCCAAACCGATCTATCCGACGTTC
 >scaffold00001_80_81_202_R
 GATCCAAGCTGTCTTAACGAGGAGTTGCC

 >scaffold00001_87_88_204_F
 GAAGTGATCACGCGATCCACGGGATTCCAC
 >scaffold00001_87_88_267_R
 AGCTTCCAGACCCTGGTCAACAGTC

 >scaffold00001_88_89_1973_F
 AGTAGCCGATGACCCCGTTGAGTTG
 >scaffold00001_88_89_463_R
 CAGGTGTTCAACCTCGCCCAGACCTTCTAC

 >scaffold00003_3_4_218_F
 ACAAGCTCATTGCCTACTAGATCGACTTCG
 >scaffold00003_3_4_1054_R
 GCATCGTCCAACGGTAGCCTTCTTTC

 >scaffold00003_7_8_365_F
 ATCCGACGAGTACGACATCCACCTG
 >scaffold00003_7_8_601_R
 CAGACCAACTACGCCACAAGCAAGG

 >scaffold00003_8_9_472_F
 CTGTTCCAGACCTTCCTCACCGTGCTTGAC
 >scaffold00003_8_9_218_R
 CTTATGCCCTGACAGAAGGTGCAAGATCAG

 >scaffold00003_10_11_1436_F
 TGGTCCTGCCGTACTTCCTGATCACCACTG
 >scaffold00003_10_11_232_R
 GATGGGACAGACTTCGTACACCGGTTG

 >scaffold00003_11_12_266_F
 CTGTATTGATGATCCACTCGCTGCCGCTC
 >scaffold00003_11_12_1184_R
 TACAGTGATCGGCCGTTGTTGGAGTGTGTG

 >scaffold00003_17_18_314_F
 TCTGAACGAGACGGAATGGGAGATCCTCAC
 >scaffold00003_17_18_222_R
 GACATCACCATCCAGGCAGAGAACATGCTG

 >scaffold00003_19_20_655_F
 GAAGGAACGGCAACTTTCGGTCACGGTAC
 >scaffold00003_19_20_485_R
 CAAGGAGACCGTCTACAAGATCTGGCATC

 >scaffold00003_23_24_235_F
 CCCAGGGAATATAACGATGGTTCAGGTCAG
 >scaffold00003_23_24_1163_R
 GAACTGCTGGTTCATCGAATGGTCGTTGAC

 >scaffold00003_24_25_268_F
 CTCGAAGTCCCGGTCAAGAAGATCCTCAC
 >scaffold00003_24_25_666_R
 TATCGTTCGCGTCGATGGCCGACAACATTG

 >scaffold00003_26_27_1270_F
 GAAATCAAGATCCTGAACCAGTCCCGGAAG
 >scaffold00003_26_27_244_R
 CCCGATTCCTTGTCCTGGAACAGATAGAAG

 >scaffold00003_27_28_833_F
 GTACTCGCCGACTTTCCCGTTCTCATC
 >scaffold00003_27_28_474_R
 GTACACCTCGTGCTCGGGACACAAATGGTC

 >scaffold00003_28_29_210_F
 GTACACGTGCCGTTCCCGTTTCTGGTGAAC
 >scaffold00003_28_29_173_R
 ACGGCGTCACGAACAACACCTACTG

 >scaffold00003_30_31_256_F
 TTCCGGGGATGAGTAGCCGACCAATTGTCC
 >scaffold00003_30_31_1239_R
 GATCACCGGTTGGTAGACCAGATCCAG

 >scaffold00003_31_32_351_F
 CACAAATTGAACTTGAGCTGGGATGTCAGG
 >scaffold00003_31_32_565_R
 CAGGATGACAGTGTGAGTCAGCCATGGAC

 >scaffold00003_35_36_707_F
 AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
 >scaffold00003_35_36_243_R
 CAGGTTGAACATCTCCTGGACCAGCGAAC

 >scaffold00003_42_43_317_F
 GTTCCTGTCCTCGTGCAGCACAATTCGATC
 >scaffold00003_42_43_298_R
 ACCGGGGATCATCGTGGTCTTGTAC

 >scaffold00003_43_44_737_F
 ATCGTTCGCAGCGGCATCGATTCAC
 >scaffold00003_43_44_992_R
 TGAAGCTGACCAAGCTGGTGGTCTC

 >scaffold00003_45_46_422_F
 GTGATGCCCTCGGATCCCAGTTCTTCGTAC
 >scaffold00003_45_46_399_R
 AACTCGGTCAAGCTCGAGTTCCTGGTG

 >scaffold00003_47_48_427_F
 CATCACGGCGAACAACAACCAGACCTAC
 >scaffold00003_47_48_271_R
 GAACAACGGCATGAGGTCCTTGAACATGAC

 >scaffold00003_48_49_532_F
 GAAGGGTCAACAGTGAGATCGCCTGGTTC
 >scaffold00003_48_49_741_R
 TGTAGCTGCCCTTCTCCTCCTGTTC

 >scaffold00003_49_50_208_F
 GTGGTGTGCATCGTGCAGATCCCGTTCTAC
 >scaffold00003_49_50_975_R
 AACCCGGAGCCGAAGAGTTCGAGAAAC

 >scaffold00003_50_51_1445_F
 TGGCGTACCTGGAGAAGGTGGTTTC
 >scaffold00003_50_51_439_R
 CAATGGCACCCTCACATATGGCAGATAATTG

 >scaffold00004_8_9_447_F
 GAACCTGCTGTTCATGGTCGGTCTG
 >scaffold00004_8_9_234_R
 ACAGTGGTCTCCGTCTACAGTCCAC

 >scaffold00004_12_13_750_F
 TCGATGGACATGCTCTCCTCCGAGATCGTC
 >scaffold00004_12_13_895_R
 TATGCGAGCGTCCGAAAAAGATCGACCAGC

 >scaffold00004_13_14_550_F
 GAGCACATCAAGATCACTGACAATGGGATC
 >scaffold00004_13_14_243_R
 AACACTCCGAAATCCGCTGGCTCTC

 >scaffold00004_14_15_556_F
 CTGGTGGGACTCGTTGAGCATCCAATG
 >scaffold00004_14_15_220_R
 CCGAGGGAAAGAACCGGACATTGTG

 >scaffold00004_15_16_1515_F
 CTTCACCGGCCTGTTCTTCGTCATCAC
 >scaffold00004_15_16_167_R
 CAGTACCTGTGGCGTCACACGCGATAAGAG

 >scaffold00004_16_17_236_F
 GGTTCGACACCAGGATTTCGAGGTCAC
 >scaffold00004_16_17_467_R
 GATCTGGTTCCACATCTGTCCCTGGTG

 >scaffold00004_18_19_226_F
 GTGTGATCGACACGGTTACGTCGAAG
 >scaffold00004_18_19_492_R
 TTAACAGGGGGCCCTTCCTTCAACACGGTC

 >scaffold00004_20_21_2075_F
 AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
 >scaffold00004_20_21_521_R
 TGAAGATCACCCAGGATCTCAAGGAGTACG

 >scaffold00004_21_22_386_F
 GAAGACTTCGCCACCGAATACCTCAGC
 >scaffold00004_21_22_182_R
 TTCGACGTACACACCACCCTCGCCGTAATC

 >scaffold00004_23_24_720_F
 TCCACCGGAACATGGGTGATACTCGAC
 >scaffold00004_23_24_552_R
 GTTGCCTTTGCACAGGTAGATCCCG

 >scaffold00004_24_25_667_F
 CAAGCCATGTCGAAGCAGCACTACTC
 >scaffold00004_24_25_322_R
 AAGCACCTGCTGTTCACAAGCGAACTGATC

 >scaffold00004_25_26_542_F
 GCAGATCGACGAGTACCTGGAGTTCTAC
 >scaffold00004_25_26_247_R
 AGGAGTCCGACCATGAAGATCAGGAAGATC

 >scaffold00004_29_30_278_F
 CATTTGTGACACCTGTCCAGCAGTACG
 >scaffold00004_29_30_264_R
 TCTGGTGAGCCGACAGGTATTCCTC

 >scf1_10_11_191_R
 ATTCCTCGCGCAGTTGACCCAGTTC
 >scf1_10_11_533_F
 TTCGCCACCACCGGTCTAGGATGAGAGATC
 
 >scf1_13_14_1221_R
 AGTCGTCGAAGCGCAGAAGGTACATGTAGG
 >scf1_13_14_961_F
 GTCATCGGACAGCCGATTCTTCGACGACTG

 >scf1_14_15_1407_F
 CTTGTTTCGATAGCATTACCGCCCAGTAAC
 >scf1_14_15_533_R
 GTACTCGTCGAAGGTGGACACGTTCACTC

 >scf1_16_17_1785_F
 CTCGAAGACGTCAACAACCACACCAATCTG
 >scf1_16_17_478_R
 CTCGTAACTCTCCATCGGTTCTCCAGTAAC

 >scf1_22_23_238_F
 GTTTCACTCATCGGTGTACCCTCCCGATC
 >scf1_22_23_244_R
 GAAGTTCGTCATCGAGAACGTCTCCGTGC

 >scf1_24_25_444_F
 ATTCGACGGGCAGGACGTCAACAAG
 >scf1_24_25_458_R
 TAGGTGTGCTCCAGGTAGGTGAGGAAC

 >scf1_26_27_369_F
 ATGAGCGAGCAGGTCTTCCTCCTAC
 >scf1_26_27_706_R
 GAACACGCCGTAGTTGTTCAGCGTGAAG

 >scf1_29_30_1643_F
 CCGATCCAGGCGAAGATCAGATTGGTC
 >scf1_29_30_767_R
 ATCGACATAGCGCTCCAACAGATCGGTGAC

 >scf1_32_33_169_R
 TTGTCAATGTAGCGTTGACGGGTGCCTGAC
 >scf1_32_33_266_F
 AACATTGACAGGGTGGCACGCAGTTCG

 >scf1_33_34_365_R
 GTTCCCTGCTGCTGAACTGGTTCTTCAC
 >scf1_33_34_795_F
 GTCGACGACGAGAATCCGGTTCATG

 >scf1_37_38_498_F
 TTCGACGAACTGTGGCAACGCTTCTCC
 >scf1_37_38_562_R
 AGGACGTCATGCACGGTCAACACAC

 >scf1_40_41_522_F
 GACTACAAGCCGGAGAACGTGCTCATCTC
 >scf1_40_41_660_R
 GTGCAGAAGGGATAGAGGGTGACGTAC

 >scf1_43_44_440_F
 CTCGGAGGAAAACGGTGAACAGTCAGCAAC
 >scf1_43_44_563_R
 TGAACGCCTCCACCCAGCATTTGATCACAG

 >scf1_44_45_485_R
 GTGTTGCGATCGGATTCGTAGCTGAC
 >scf1_44_45_93801_F
 ACACTGCTGCCGCCACGGTTGACTGT

 >scf1_46_47_370_F
 GTAAACTCCTGATCTCAGCCGCTCTCGTG
 >scf1_46_47_845_R
 CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG

 >scf1_53_54_414_F
 TTCTGGAAGACCATCTGCAGACCGATCTCG
 >scf1_53_54_823_R
 TACGCGAAGGGACGTTAGAGATGGCAC

 >scf1_54_55_1250_F
 CTGGAACCGGATGATGTTGGAGTCCTC
 >scf1_54_55_175_R
 TACCGAGGAACTGATCGACCTCTCC

 >scf1_56_57_184_R
 CAAACCGGTGGTCGGGCTGATCATGTATTC
 >scf1_56_57_412_F
 GTACTCACGATGGTCCTTCTCCTCGTTG

 >scf1_59_60_1979_F
 GTGACCAGCCAGGTCTGTTCGAATCTCAC
 >scf1_59_60_625_R
 GAGATCATGTACGCCGACTTCAGCTTCGTG

 >scf1_63_64_1286_R
 CGATAGGTGAGACTCGATGCATGGTTAGTG
 >scf1_63_64_711_F
 ACGTAGTTCCCGACCGTAGAGCAGTTC

 >scf1_66_67_346_R
 ACTGTTCCTGCTGTACTCCTCGGTG
 >scf1_66_67_428_F
 CTTCGTCACTGGCTGTGTCAAGGTCAAC

 >scf1_6_7_870_R
 CGATCTCCACGTTTGACAGGATGCTCTG
 >scf1_6_7_939_F
 ATCAGCGGTTACGTCTGGTGGTGAC

 >scf1_7_8_545_R
 ATCAGTTTGATCGGCGAGACGTGCGAGACC
 >scf1_7_8_818_F
 GAACTGGAGTTGATGGAGCAGCGCGAAAC

 >scf3_12_13_2070_F
 CTCCAGCAGGGTTTCATCGATTCGGTAG
 >scf3_12_13_324_R
 CTGGACCAGTTCACCCTCCAGATCATG

 >scf3_18_19_471_R
 GTATACCGCAACATCGGCGAAATGAAGTC
 >scf3_18_19_746_F
 GTTGCTTCGAATCTGGTGACGGGAG

 >scf3_20_21_200_R
 GAAGATCGGCAGCTCCATCGTCTCATC
 >scf3_20_21_294_F
 ATCAGCCCCGAGCAATTGACCGACCTGAAC

 >scf3_21_22_1034_F
 GAAGTAACCGGTGGTGTTTTCGGCCAG
 >scf3_21_22_354_R
 ATCTCGCCGGTTACAAGCAGATCCTC

 >scf3_22_23_1299_F
 TTCAGATGGTTAAGGACAGCTTCATGAGCG
 >scf3_22_23_368_R
 ACGTGATCGGTGGGAACTTCGACTC

 >scf3_25_26_360_F
 GAAAGGCTGACCATCTACCCGGAGTAC
 >scf3_25_26_636_R
 TGATCCACGTGTCCGTACATCATCGTG

 >scf3_33_34_1969_F
 GTCCTCAAGCATCGCAAGCGCAAGGAAAAG
 >scf3_33_34_485_R
 CTCCAACTGCTCGGAGAGGAACTTGTAAC

 >scf3_34_35_222_R
 ATCTCCGCAGTCATATCGGTACGGG
 >scf3_34_35_491_F
 GAACGAGACCAGGAGACATAGTGGCGAGTC

 >scf3_36_37_355_F
 CTGCTCTTCCTCGGCATGCTCATGTTC
 >scf3_36_37_430_R
 ATAGCCCGCCCACTGCATGACAAAC

 >scf3_38_39_613_R
 GTACGAGCAGTTCACCAGTTGCGTG
 >scf3_38_39_791_F
 AACTGTCGACGAAGAACCGCGTCATGAGAG

 >scf3_39_40_333_R
 CTGATCCGTCATGGCGCACATTCCACGATG
 >scf3_39_40_351_F
 CACTTGTCTTCTCCTCCGGATACCTGG

 >scf3_44_45_1117_F
 CTCTGTGAGTACGTGGAACAGGCGATG
 >scf3_44_45_224_R
 AAGATCACCACGGCGTCGACACAGCTCAAC

 >scf3_6_7_245_R
 GCATGCTGTTGTTGATGCTGTCCTTCAG
 >scf3_6_7_918_F
 ATGCTCAGTCCGGGAGTACAGCTCGGTAAC

 >scf3_9_10_212_R
 TTTCCGTTGAGCCGCCACTCGTACTTGTAG
 >scf3_9_10_376_F
 CATGCACAAGCAGGACCTCATCGAG

 >scf4_19_20_273_F
 GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC
 >scf4_19_20_878_R
 GACGAGCAACTGGACTCGCTGAACAAG

 >scf4_26_27_255_F
 GATCGTTCGACTACCCACAATCCGCAGATC
 >scf4_26_27_671_R
 GGTAGAGCAGCCATTCCAGTTCCTGTG

 >scf4_27_28_485_R
 GAAAACGGGGTGTTCCAGCTTCATCGCTTC
 >scf4_27_28_577_F
 CTGTTCACCGAGGAGGGCGAGTTCTTCTTC

 >scf4_28_29_1409_F
 AACCGACACACCTGCAGTTGCCGTACCAAC
 >scf4_28_29_207_R
 TACTCCGGGATAAGCAAGGCTGAGCTG

 >scf4_30_31_444_F
 ACCTTCTTGCTCTTACGGCCCACCTTG
 >scf4_30_31_739_R
 GAGTACGTCAAGTTCATTTTCGCCACCAC

 >scf3_1_2_276_F
 GACGTGTACATCTCGAACATCTCGCGG
 >scf3_1_2_387_R
 AAAACGGGCGTTCACCGCATGGTAC