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(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)- 07:51, 20 July 2014 14.5.2 (hist) [40 bytes] Guest (Talk | contribs) (Created page with "Oneshot-gibson product : electroporation")
- 07:50, 20 July 2014 14.5.1 (hist) [61 bytes] Guest (Talk | contribs) (Created page with "2ml 접종한것 nutrient broth 100ml에 배양 -안자라..")
- 07:50, 20 July 2014 14.4.29 (hist) [71 bytes] Guest (Talk | contribs) (Created page with "''Corynebacterium glutamicum'' comp cell 만들기 -2 ml LB 에 접종")
- 07:49, 20 July 2014 14.4.28 (hist) [43 bytes] Guest (Talk | contribs) (Created page with "25일에 gibson transformation한거 접종")
- 07:48, 20 July 2014 14.4.25 (hist) [119 bytes] Guest (Talk | contribs) (Created page with "gibson assembly 다시 (2* master mix) 후 transformation : 콜로리 떳다. pCLTon2 tranformation -안되...엉엉")
- 07:46, 20 July 2014 14.4.24 (hist) [248 bytes] Guest (Talk | contribs) (Created page with "1. streptomycin LB plate 만들기 2. gibson assembly(2* master mix) experiment : pilin 9 : pEKEx2 1 + mix 10 control(positive) : 5 + mix 5 assembly 된거 바로 ''E.coli'' ...")
- 07:41, 20 July 2014 14.4.16 (hist) [639 bytes] Guest (Talk | contribs) (Created page with "PCR srt A gene cluster for gibson assembly with Q5 pol PCR pEKEx2 for gibson assembly with Q5 pol template : genomic DNA, pEKEx2 <조성> rxn buffer 10 dNTP 1 F.primer 2.5 ...")
- 07:34, 20 July 2014 14.4.15 (hist) [320 bytes] Guest (Talk | contribs) (Created page with "PCR srt A gene cluser for gibson assembly and cloning into pSB1C3 with Q5 pol template <조성> rxn buffer 10 dNTP 1 F.primer 2.5 R.primer 2.5 Template 2 Q5 pol 0.5 Q...")
- 07:32, 20 July 2014 14.4.14 (hist) [259 bytes] Guest (Talk | contribs) (Created page with "PCR srt A gene cluster for gibson assembly with Q5 pol <조성> rxn buffer 10 dNTP 1 F.primer 2.5 R.primer 2.5 Template 2 Q5 pol 0.5 Q5 GC 10 DW 22.5 up to 50 <conditi...")
- 07:29, 20 July 2014 14.4.11 (hist) [398 bytes] Guest (Talk | contribs) (Created page with "1.pSB1C3 다시 접종 2. 10일에 gel prep 한 srt A gene cluster 를 template로 PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol PCR for pEKEx2-srtA gene cl...")
- 13:09, 24 June 2014 14.4.12 (hist) [13 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 추출")
- 08:40, 23 June 2014 14.4.10 (hist) [569 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 다시 접종 PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol")
- 08:34, 23 June 2014 14.4.8 (hist) [378 bytes] Guest (Talk | contribs) (Created page with "보다 증폭잘된다는 TAKARA LA Taq 사용 1. srtA gene cluster for pSB1C3 cloning PCR with LA Taq <조성> LA Taq 0.5 Rxn buffer 5 MgCl2 5 dNTP 8 F,R Primer 4 Templa...")
- 08:29, 23 June 2014 14.4.7 (hist) [297 bytes] Guest (Talk | contribs) (Created page with "srtA gene cluster for pSB1C3 cloning PCR with Q5 pol <조성> Q5 rxn buffer : 10 dNTP : 1 F.R primer : 2.5 Template : 2 Q5 pol : 0.5 Q5 GC : 10 DW : 21.5 up to 50 < P...")
- 08:24, 23 June 2014 14.4.2 (hist) [80 bytes] Guest (Talk | contribs) (Created page with "pSB1c3-srtA gene cluster cloning 할 때 쓸 srtA gene cluster PCR primer 주문")
- 08:22, 23 June 2014 14.4.1 (hist) [24 bytes] Guest (Talk | contribs) (Created page with "srtA gene cluster 확인")
- 08:22, 23 June 2014 14.3.31 (hist) [26 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 , pEKEx2 size check")
- 08:21, 23 June 2014 14.3.29 (hist) [21 bytes] Guest (Talk | contribs) (Created page with "pEKEx2 plasmid 추출")
- 08:19, 23 June 2014 14.3.28 (hist) [64 bytes] Guest (Talk | contribs) (Created page with "Oneshot-pEKEx2 : LB 3ml 접종 Oneshot-pCLTon2 : transformation Agar")
- 08:18, 23 June 2014 14.3.27 (hist) [86 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 plasmid 추출 Oneshot-pEKEx2 : transformation Spectinomycin 배지 만들기")
- 08:17, 23 June 2014 14.3.26 (hist) [273 bytes] Guest (Talk | contribs) (Created page with "Oneshot-pSB1C3 : LB 3ml 접종 Uniprot에서 Agarase pili에 적합한 agarase 알아보기 N,C terminal 쪽에 catabolytic domain 없이 잘 빠져나와있고, monomer에 b-...")
- 08:13, 23 June 2014 14.3.25 (hist) [50 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 증폭위해 Oneshot-pSB1C3 transformation")
- 02:10, 2 April 2014 2014 ComGen Course (hist) [788 bytes] Mbnmbn00 (Talk | contribs) (Created page with "=2014 spring Comgen Course= ==Info== Textbook: :[http://www.amazon.com/Biological-Sequence-Analysis-Probabilistic-Proteins/dp/0521629713] Biological Sequence Analysis Lecture: :[...")
- 12:16, 26 March 2014 Agarse screening (hist) [144 bytes] Guest (Talk | contribs) (Created page with "http://www.uniprot.org/uniprot/?query=job%3a2014032662AJZWU6QE&format=tab&columns=id,entry%20name,reviewed,protein%20names,genes,organism,length")
- 04:18, 17 March 2014 Taq 정제방법 (hist) [220 bytes] Guest (Talk | contribs) (Created page with "sonification 해서 원심분리 후 soup만 75도에서 1시간 가열하면 Taq말고 다른 대장균단백질은 다 aggrigation되서 가라앉는다 가열 후 soup만 c...")
- 04:16, 17 March 2014 14.3.13 (hist) [64 bytes] Guest (Talk | contribs) (Created page with "호구 GFP 희석않고 reaction test 했더니 다 끌림...")
- 04:15, 17 March 2014 14.3.12 (hist) [29 bytes] Guest (Talk | contribs) (Created page with "11일에 배양한 GFP 정제")
- 04:15, 17 March 2014 14.3.11 (hist) [114 bytes] Guest (Talk | contribs) (Created page with "10일에 접종한거 AIM에 배양 호구 GFP로 reaction test 했으나 GFP가 생각보다 농도가 옅다??")
- 04:14, 17 March 2014 14.3.10 (hist) [31 bytes] Guest (Talk | contribs) (Created page with "Bl21-2*Ala:GFP만 다시 접종")
- 04:13, 17 March 2014 14.3.6 (hist) [78 bytes] Guest (Talk | contribs) (Created page with "GFP 10배 희석, mRFP 30배 희석해서 PAGE걸었는데 gfp가 이상하다")
- 04:13, 17 March 2014 14.3.5 (hist) [70 bytes] Guest (Talk | contribs) (Created page with "4일에 농축한것 정량 srtA-2.09 mg/ml GFP-25.1 mRFP-63.5-74.4")
- 04:12, 17 March 2014 14.3.4 (hist) [39 bytes] Guest (Talk | contribs) (Created page with "새로 정제한 srtA, GFP,mRFP 농축")
- 04:12, 17 March 2014 14.2.26 (hist) [204 bytes] Guest (Talk | contribs) (Created page with "25일에 induction한거 cell harvest, sonification, centrifuge 후 soup만 75도에서 1시간 가열, 원심분리 후 soup만 centricon으로 농축 - Taq 정제 25일에 ...")
- 04:10, 17 March 2014 14.2.25 (hist) [77 bytes] Guest (Talk | contribs) (Created page with "24일에 접종한거 1L large scale up 후 induction streaking한거 접종")
- 04:09, 17 March 2014 14.2.24 (hist) [93 bytes] Guest (Talk | contribs) (Created page with "Taq Pol DIY로 만들기 프로젝트 Taq 클로닝 된거 plate에 streaking lb에 접종")
- 04:08, 17 March 2014 14.2.21 (hist) [27 bytes] Guest (Talk | contribs) (Created page with "srtA,mRFP,GFP 다시 정제")
- 04:08, 17 March 2014 14.2.18 (hist) [118 bytes] Guest (Talk | contribs) (Created page with "17일 실험 다시해서 젤 내려봄 assembly 확인 위해 transformation control이 이상해서 망함..")
- 04:06, 17 March 2014 14.2.17 (hist) [102 bytes] Guest (Talk | contribs) (Created page with "Gibson assembly (one-step) 실험 - 직접 만든 master mixure 잘되나 실험 잘 모르겠어..")
- 04:05, 17 March 2014 14.2.14 (hist) [57 bytes] Guest (Talk | contribs) (Created page with "control 용량을 높여서 다시내렸지만 이상함")
- 04:05, 17 March 2014 14.2.12 (hist) [26 bytes] Guest (Talk | contribs) (Created page with "10일꺼 젤 다시 내림")
- 04:04, 17 March 2014 14.2.10 (hist) [121 bytes] Guest (Talk | contribs) (Created page with "Reaction test BSA SrtA GFP RFPwaste Marker B+G+R S+G+R(30m,1h,2h,3h) 젤이 이상해...")
- 04:03, 17 March 2014 14.2.6 (hist) [200 bytes] Guest (Talk | contribs) (Created page with "standard curve 다시 쟤서 단백질 정량 srtA : 0.76-1.05 mg/ml GFP : 20.6 mRFP : 63.48-65.14 mRFP waste : 5.8 몰비율 맞춰서 대충희석해서 다음에 1대1 r...")
- 04:01, 17 March 2014 14.2.5 (hist) [94 bytes] Guest (Talk | contribs) (Created page with "4일에 만든 standard curve로 정량 후 제대로 희석해서 PAGE 내렸으나 이상함")
- 04:00, 17 March 2014 14.2.4 (hist) [113 bytes] Guest (Talk | contribs) (Created page with "1. bradford정량으로 srtA, GFP,mRFP 확인해 보려고 일단 standard curve만듦 2. Double transformation")
- 03:57, 17 March 2014 14.2.3 (hist) [104 bytes] Guest (Talk | contribs) (Created page with "29일ㅇ[ 내린것 많아서 다 끌림 5배 희석 후 로딩 2 5 52 52 52 근데 이것도 끌림..")
- 07:06, 29 January 2014 14.1.29 (hist) [167 bytes] Guest (Talk | contribs) (Created page with "28일에 내린 15% SDS-PAGE gel 결과가 좋지 않아서 다시 내림 단백질이 너무많고, 오래 내려서 그런 것 같아서, 2람다만 내리기로함")
- 07:25, 28 January 2014 14.1.28 (hist) [360 bytes] Guest (Talk | contribs) (Created page with "30과 10의 centricon을 이용한 단백질 농축 및 buffer change 2*Ala-GFP, mRFP1-LPETAA, srtA 농축 및 srt reaction buffer change 후 15% SDS-PAGE gel로 내려봄 ;GFP...")
- 06:59, 28 January 2014 14.1.24 (hist) [81 bytes] Guest (Talk | contribs) (Created page with "23일에 정제한 16도 AIM, SDS-PAGE gel 내리기 -> 성공했다고 합니다")
- 06:58, 28 January 2014 14.1.23 (hist) [47 bytes] Guest (Talk | contribs) (Created page with "21일에 induction한 16도꺼 단백질 정제")
- 06:58, 28 January 2014 14.1.22 (hist) [144 bytes] Guest (Talk | contribs) (Created page with "21일에 induction한 것 단백질정제 전에 bugbuster로 발현확인테스트 후 25도꺼만 단백질 정제 후 SDS-PAGE gel 내리기")