Strains

From CSBLwiki

Contents 1 Stock center 1.1 Korea 1.2 USA 2 Stocks@KUCSBL 3 Strains info.

Stock center

Korea

• Stock centers: KACC, KCTC

USA

• Stock centers: ATCC, CGSC

Stocks@KUCSBL

• E. coli
  • DH5a
  • XL1-Blue
  • SURE
  • DH10B
    • While constitutive expression of the red gamma gene has a toxic effect in DH10B (recA-) cells under some conditions, thus limiting the efficiency of recombination, tightly regulated expression of the gamma gene together with simultaneous expression of the red alpha and beta genes allows efficient homologous recombination between linear DNA fragments and BACs resident in such cells as DH10B [1]
  • TOP10
• CGSC strains (Apr 2010 purchased at $50)

1. 8175 (BW19851): RP4-2
2. 7629 (BT340): pCP20
3. 7635 (BW25113)
   1. E. coli K12
   2. Keio Collection host strain for gene deletion
4. 7636 (BW25141)
5. 5478 (K27): fadD88
6. 7633 (BW25113/pKD46): lost
7. 7739 (BW25141/pKD13): lost

• ordered on 9/20/2010

1. #7857 BW5328/pAH69
2. #7845 BW23474/pAH70
3. #7858 BW23473/pAH83
4. #7862 BW23473/pAH129
5. #7861 BW5328/pAH123
6. #7869 BW23474/pAH153
7. #12353 C600/RK2
8. #9796 JW2227
9. #7633 BW25141/pKD13
10. #7631 BW25141/pKD3
11. #7988 BW25141/pKD32
12. #7632 BW25141/pKD4

Strains info.

• E. coli genotypes - in openwetware@MIT
• Novagen E. coli manual - Novagen Competent Cells
• Choosing a competent E. coli strain
• Invitrogen Comp. Cell::Genotype]