Molecular biology

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	**[http://www.strgen.org/protocols/lic-rxn-transf.pdf LIC transformation]		**[http://www.strgen.org/protocols/lic-rxn-transf.pdf LIC transformation]
	*Electrocompetent cell [[media:ElectrocompetentEcoli.pdf|Protocol (PDF)]]		*Electrocompetent cell [[media:ElectrocompetentEcoli.pdf|Protocol (PDF)]]
		+	*[[Concentration of antibiotics commonly used]]

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Revision as of 16:44, 12 February 2008

• Commonly used reagents and equipment in molecular biology (PDF)
• PCR - General rules for primer design
• Genomic DNA extraction
• Competent cell preparation BSGC protocol (PDF)
• LIC cloning introduction LIC introduction - Ligation Independent Cloning
• LIC cloning protocol (Berkeley Structural Genomics Center)
  • Vector preparation
  • Insert preparation
  • LIC transformation
• Electrocompetent cell Protocol (PDF)
• Concentration of antibiotics commonly used