E. coli knockout

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E. coli Gene Replacement

Lambda red recombinase method

Materials

plasmids 
 pKD46: Coding lambda red recombinase  
 pKD13: Kanamycin resistance gene template plasmid 
 pCP20: Flipase system  
Reagents 
 L-arabinose: lambda red recombinase pBAD promoter inducer
Equipment 
 Incubators (30°C and 37°C) 
 Electroporator