E. coli knockout

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E. coli Gene Replacement

Lambda red recombinase method

Materials

• plasmids
  • pKD46: Coding lambda red recombinase
  • pKD13: Kanamycin resistance gene template plasmid
  • pCP20: Flipase system
• Reagents
  • L-arabinose: lambda red recombinase pBAD promoter inducer
• Equipment
  • Incubators (30°C and 37°C)
  • Electroporator