14.4.10
From CSBLwiki
pSB1C3 다시 접종
PCR for pSB1c3-srtA gene cluster cloning with Q5 pol PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol
<조성>
Q5 Rxn buffer 10
dNTP 1
F,R primer 2.5
Template 2 ->srtA gene cluster, pEKEx2
Q5 pol 0.5
Q5 GC 10
DW 21.5
up to 50
<PCR condition>
3-step, Touchdown 조건 안적어놔서 모름
3-step으로 "PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol" pEKEx2랑 srtA gene cluser 성공 -> srtA gene cluster gel prep
Touchdown으로 "PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol" pEKEx2성공