14.4.14
From CSBLwiki
PCR srt A gene cluster for gibson assembly with Q5 pol
template : genomic DNA
<조성>
rxn buffer 10
dNTP 1
F.primer 2.5
R.primer 2.5
Template 2
Q5 pol 0.5
Q5 GC 10
DW 22.5
up to 50
<condition>
98 30
-98 10
68 15'-*30
72 10'
16 --
실패
