14.4.15
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PCR srt A gene cluser for gibson assembly and cloning into pSB1C3 with Q5 pol
template - genomic DNA and 4/11에 prep 한 product
<조성>
rxn buffer 10
dNTP 1
F.primer 2.5
R.primer 2.5
Template 2
Q5 pol 0.5
Q5 GC 10
DW 22.5
up to 50
<condition>
98 30
-98 10
60 30
72 5'-*35
72 10'
16 --
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