14.4.24

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1. streptomycin LB plate 만들기

2. gibson assembly(2* master mix)

experiment : pilin 9 : pEKEx2 1 + mix 10

control(positive) : 5 + mix 5

assembly 된거 바로 E.coli oneshot 에 transformation

3. Oneshot-pCLTon2 transformation : 실패

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