14.4.7
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srtA gene cluster for pSB1C3 cloning PCR with Q5 pol
<조성>
Q5 rxn buffer : 10
dNTP : 1
F.R primer : 2.5
Template : 2
Q5 pol : 0.5
Q5 GC : 10
DW : 21.5
up to 50
< PCR condition>
66,68,70 gradient, anealing 2 step. 기록을 안해놔서 모름...
Taq 증폭 약해서 실패한듯?
