From CSBLwiki

Contents 1 Mode of actions 1.1 Accessibility 1.1.1 Endoglucanse 1.1.2 Exoglucanase 1.1.3 Mixed 1.2 Processivity 1.3 Synergism 1.4 Substrates 1.4.1 Crystalline cellulose 1.4.2 Non-crystalline cellulose 1.4.3 etc. 2 CAZy GH families

Mode of actions

• (Fungus) "The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose."[1]

Accessibility

Endoglucanse

• e.g. T. reesei EG-1; GH7; CBM1

Exoglucanase

• Reducing end acting exoglucanase (CBH I) from T. reesei; GH7; CBM1
• Non-reducing end acting (CBH II); GH6; CBM1

Mixed

Processivity

Synergism

1. Error fetching PMID 7980450: [Synergism1]

Substrates

Crystalline cellulose

Non-crystalline cellulose

etc.

CAZy GH families

CAZy class	Taxonomic distribution	Gene name	Mode	Clan	Typical organisms
GH5	A,B,E	Cellulase family A		GH-A	Saccharophagus,Clostridium
GH6	B,E	CBH-II, Cellulase family B	non-reducing end(exo)		Chrysosporium,Trichoderma,Saccharophagus
GH7	E	EG-I, CBH-I, Cellulase family C	reducing end(exo)	GH-B
GH8	B	Cellulase family D	Endo-, Exo-	GH-D
GH9	A,B,E	Cellulase family E	Endo-
GH44	B(E)	Cellulase family J	Endo-		Clostridium
GH48	B,E		Endo-, Exo-(reducing end)		Clostridium,Hahella