Evolutionary age

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	*Species in Pfam (NCBI taxonomy): 148,925 species		*Species in Pfam (NCBI taxonomy): 148,925 species
	*Genus (taxonomy): 31,949		*Genus (taxonomy): 31,949
		+	**taxonomy - browsing order in the web?
	===Procedure===		===Procedure===

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Revision as of 05:25, 14 August 2010

Contents 1 Evolutionary age of protein domains 1.1 Pfam data (24.0) 1.2 Tree data 1.3 Results 1.4 Procedure

Evolutionary age of protein domains

(Based on this reference)

1. Error fetching PMID 16959887: [Reference]

Pfam data (24.0)

• The Pfam database
  • ftp current_release
  • User manual (the pfam format)
  • Tip: use Mysql dump <- easy to handle the content

# download total DB (estimated ~2 days)
wget -c ftp://ftp.sanger.ac.uk/pub/databases/Pfam/current_release/database.tar &

• Loading into MySQL
  • Not all DBs were imported (only a few key DBs for this study)
  • pfamseq, genome_seqs, ncbi_taxonomy, genome_species, pfamA, gene_ontology

mysql -u user -p
mysql>create DATABASE pfam24; \q
mysql -u user -p pfam24 < FULL_PATH/pfamseq.sql
mysql -u user -p
mysql>use pfam24
mysql>load data local infile 'pfamseq.txt' into table pfamseq FIELDS ENCLOSED BY "\'";

• MySQL로 로딩시, 테이블 작성 순서대로 할것 - 에러발생 (작성하지 않은 테이블의 키인덱스 링크 관련 키워드)
  • loading time: a few hours
• Loading data file in background job

mysql -u user -p'passwd' database < loadscript.sql &

Tree data

• • Silva: SSU rRNA database

Results

• Species in Pfam (NCBI taxonomy): 148,925 species
• Genus (taxonomy): 31,949
  • taxonomy - browsing order in the web?

Procedure

• Extract the taxonomic origins of each protein
• Get the Taxonomy info. of each origin
• Non-redundant (NR) set of taxonomic origins
• Collect all Small Subunit (SSU) rRNA sequences of the NR set
• Build the universal tree of life using SSU sequences
• Mapping the each protein belonging to a given domain into the universal tree
• Check which node is the most recent common ancestor (MRCA) node
• Calculate the branch length between MRCA and LCA (last common ancestor)