Genome finishing

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		+	==PCR==
		+	===Arrange Scaffold===
		+
		+	===Sequencing===
		+	7head-3head
		+	1tail-3head - (7head-3head)
		+
	==Reference==		==Reference==
	*[http://mccombielab.cshl.org/FINS/finishing_overview.pdf Finishing Overview] - [[media:finishingoverview.pdf|PDF]]		*[http://mccombielab.cshl.org/FINS/finishing_overview.pdf Finishing Overview] - [[media:finishingoverview.pdf|PDF]]

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Revision as of 00:49, 16 August 2010

Contents 1 PCR 1.1 Arrange Scaffold 1.2 Sequencing 2 Reference

PCR

Arrange Scaffold

Sequencing

7head-3head 1tail-3head - (7head-3head)

Reference

• Finishing Overview - PDF
• Consed--A Finishing Package (Editor, Autofinish, Autoreport, Autoedit, and Align Reads To Reference Sequence)
• Genomics Education - see some tutorials for finishing with Consed
• Automated Finishing with Autofinish
• Finishing genomes with limited resources: lessons from an ensemble of microbial genomes
• Google Search Result: genome+sequencing+finishing+pcr+protocol