Genome finishing

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Revision as of 00:49, 16 August 2010 (view source) Csbl (Talk | contribs) ← Older edit		Revision as of 00:54, 16 August 2010 (view source) Csbl (Talk | contribs) (→PCR) Newer edit →
Line 1:		Line 1:
	==PCR==		==PCR==
	===Arrange Scaffold===		===Arrange Scaffold===
		+
	===Sequencing===		===Sequencing===
-	7head-3head	+	#5tail-8head: ok
-	1tail-3head - (7head-3head)	+	#8tail-8head (8tail: fail(noisy))
		+	#7head-3head
		+	#1tail-3head: 7head-3head
	==Reference==		==Reference==

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Revision as of 00:54, 16 August 2010

Contents 1 PCR 1.1 Arrange Scaffold 1.2 Sequencing 2 Reference

PCR

Arrange Scaffold

Sequencing

1. 5tail-8head: ok
2. 8tail-8head (8tail: fail(noisy))
3. 7head-3head
4. 1tail-3head: 7head-3head

Reference

• Finishing Overview - PDF
• Consed--A Finishing Package (Editor, Autofinish, Autoreport, Autoedit, and Align Reads To Reference Sequence)
• Genomics Education - see some tutorials for finishing with Consed
• Automated Finishing with Autofinish
• Finishing genomes with limited resources: lessons from an ensemble of microbial genomes
• Google Search Result: genome+sequencing+finishing+pcr+protocol