Genome finishing

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Revision as of 00:54, 16 August 2010 (view source) Csbl (Talk | contribs) (→PCR) ← Older edit		Revision as of 01:03, 16 August 2010 (view source) Csbl (Talk | contribs) (→PCR) Newer edit →
Line 4:		Line 4:
	===Sequencing===		===Sequencing===
		+	*Primer: Product
	#5tail-8head: ok		#5tail-8head: ok
-	#8tail-8head (8tail: fail(noisy))	+	#8tail-8head: 4tail-8head
	#7head-3head		#7head-3head
	#1tail-3head: 7head-3head		#1tail-3head: 7head-3head

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Revision as of 01:03, 16 August 2010

Contents 1 PCR 1.1 Arrange Scaffold 1.2 Sequencing 2 Reference

PCR

Arrange Scaffold

Sequencing

• Primer: Product

1. 5tail-8head: ok
2. 8tail-8head: 4tail-8head
3. 7head-3head
4. 1tail-3head: 7head-3head

Reference

• Finishing Overview - PDF
• Consed--A Finishing Package (Editor, Autofinish, Autoreport, Autoedit, and Align Reads To Reference Sequence)
• Genomics Education - see some tutorials for finishing with Consed
• Automated Finishing with Autofinish
• Finishing genomes with limited resources: lessons from an ensemble of microbial genomes
• Google Search Result: genome+sequencing+finishing+pcr+protocol