Genome finishing
From CSBLwiki
(Difference between revisions)
(→Arrange Scaffold) |
(→Arrange Scaffold) |
||
Line 4: | Line 4: | ||
** 8-1 | ** 8-1 | ||
** 8-6 | ** 8-6 | ||
- | ** | + | ** 8-7-3-8 |
- | + | ||
** 8-2-8 | ** 8-2-8 | ||
** 8-5-8 | ** 8-5-8 |
Revision as of 01:06, 16 August 2010
Contents |
PCR
Arrange Scaffold
- Pieces
- 8-1
- 8-6
- 8-7-3-8
- 8-2-8
- 8-5-8
- 8-4-8
Sequencing
- Primer: Product
- 5tail-8head: ok
- 8tail-8head: 4tail-8head
- 7head-3head
- 1tail-3head: 7head-3head
Reference
- Finishing Overview - PDF
- Consed--A Finishing Package (Editor, Autofinish, Autoreport, Autoedit, and Align Reads To Reference Sequence)
- Genomics Education - see some tutorials for finishing with Consed
- Automated Finishing with Autofinish
- Finishing genomes with limited resources: lessons from an ensemble of microbial genomes
- Google Search Result: genome+sequencing+finishing+pcr+protocol