Genome finishing

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Revision as of 01:09, 16 August 2010 (view source) Csbl (Talk | contribs) (→Sequencing) ← Older edit		Revision as of 01:25, 16 August 2010 (view source) Csbl (Talk | contribs) (→Arrange Scaffold) Newer edit →
Line 4:		Line 4:
	** 8-1		** 8-1
	** 8-6		** 8-6
-	** 8-7-3-8	+	** 8-7-3(R)-8
	** 8-2-8		** 8-2-8
	** 8-5-8		** 8-5-8

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Revision as of 01:25, 16 August 2010

Contents 1 PCR 1.1 Arrange Scaffold 1.2 Sequencing 2 Reference

PCR

Arrange Scaffold

• Pieces
  • 8-1
  • 8-6
  • 8-7-3(R)-8
  • 8-2-8
  • 8-5-8
  • 8-4-8

Sequencing

• Primer: Product

1. 5tail-8head: ok
2. 8tail-8head: 4tail-8head
3. 1tail-3head: 7head-3head - 1tail primer의 중간부터 끝 부분이 7 head에 붙은 것으로 생각됨.
4. 7head-3head

Reference

• Finishing Overview - PDF
• Consed--A Finishing Package (Editor, Autofinish, Autoreport, Autoedit, and Align Reads To Reference Sequence)
• Genomics Education - see some tutorials for finishing with Consed
• Automated Finishing with Autofinish
• Finishing genomes with limited resources: lessons from an ensemble of microbial genomes
• Google Search Result: genome+sequencing+finishing+pcr+protocol