Genome finishing

From CSBLwiki

Contents 1 PCR 1.1 Arrange Scaffold 1.2 Sequencing 2 Reference

PCR

Arrange Scaffold

• Pieces
  • 8-1
  • 8-6
  • 3-8
  • 7-8
  • 8-2-8
  • 8-5-8
  • 8-4-8

Sequencing

• Primer: Product

1. 5tail-8head: ok
2. 8tail-8head: 4tail-8head
3. 7head-3head
4. 1tail-3head: 7head-3head

Reference

• Finishing Overview - PDF
• Consed--A Finishing Package (Editor, Autofinish, Autoreport, Autoedit, and Align Reads To Reference Sequence)
• Genomics Education - see some tutorials for finishing with Consed
• Automated Finishing with Autofinish
• Finishing genomes with limited resources: lessons from an ensemble of microbial genomes
• Google Search Result: genome+sequencing+finishing+pcr+protocol