Knockout1
From CSBLwiki
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==== Materials ==== | ==== Materials ==== | ||
*'''Plasmids''' | *'''Plasmids''' | ||
- | + | **pKD46 | |
- | + | *** | |
- | + | **pKD13 | |
- | + | *** | |
- | + | **pCP20 | |
+ | *** | ||
+ | *'''Reagents''' | ||
+ | **L-arabinose | ||
+ | *'''Equipment''' | ||
+ | ** | ||
==== Procedure ==== | ==== Procedure ==== |
Revision as of 02:38, 13 October 2010
Gene replacement
- target gene: yfaL
Materials
- Plasmids
- pKD46
- pKD13
- pCP20
- pKD46
- Reagents
- L-arabinose
- Equipment