MiSeq
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(Created page with "==DNA quality== ==RNAseq== ;Sample RNA amounts :Total RNA >10ug (concentration: >200 ng/ul) :mRNA >1ug (concentration: >200 ng/ul) ;Sample RNA quality :Intact rRNA should be obs...")
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(Created page with "==DNA quality== ==RNAseq== ;Sample RNA amounts :Total RNA >10ug (concentration: >200 ng/ul) :mRNA >1ug (concentration: >200 ng/ul) ;Sample RNA quality :Intact rRNA should be obs...")
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Revision as of 04:29, 13 November 2012
DNA quality
RNAseq
- Sample RNA amounts
- Total RNA >10ug (concentration: >200 ng/ul)
- mRNA >1ug (concentration: >200 ng/ul)
- Sample RNA quality
- Intact rRNA should be observed in agarose gel electrophoresis total RNA preparation
- A260/280 ratio >1.8
- A260/230 ratio >1.8
