MiSeq

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Revision as of 04:29, 13 November 2012 (view source) Igchoi (Talk | contribs) (→DNA quality) ← Older edit		Revision as of 04:30, 13 November 2012 (view source) Igchoi (Talk | contribs) Newer edit →
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-	==genomic DNA sequencing==	+	==Next generation sequencing==
-	==RNAseq==	+	===Genomic DNA sequencing===
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		+	===RNAseq===
	;Sample RNA amounts		;Sample RNA amounts
	:Total RNA >10ug (concentration: >200 ng/ul)		:Total RNA >10ug (concentration: >200 ng/ul)

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Revision as of 04:30, 13 November 2012

Next generation sequencing

Genomic DNA sequencing

RNAseq

Sample RNA amounts

Total RNA >10ug (concentration: >200 ng/ul)

mRNA >1ug (concentration: >200 ng/ul)

Sample RNA quality

Intact rRNA should be observed in agarose gel electrophoresis total RNA preparation

A260/280 ratio >1.8

A260/230 ratio >1.8