MiSeq

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	==Useful links==		==Useful links==
-	;[http://support.illumina.com/sequencing/sequencing_instruments/miseq/questions.ilmn| MiSeq Q&A]	+	;*[http://support.illumina.com/sequencing/sequencing_instruments/miseq/questions.ilmn| MiSeq Q&A]

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Revision as of 04:34, 13 November 2012

Contents 1 Next generation sequencing 1.1 Genomic DNA sequencing 1.2 RNAseq 2 Useful links

Next generation sequencing

Genomic DNA sequencing

RNAseq

Sample RNA amounts

Total RNA >10ug (concentration: >200 ng/ul)

mRNA >1ug (concentration: >200 ng/ul)

Sample RNA quality

Intact rRNA should be observed in agarose gel electrophoresis total RNA preparation

A260/280 ratio >1.8

A260/230 ratio >1.8

Useful links

• MiSeq Q&A