MiSeq
From CSBLwiki
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(→DNA sequencing) |
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===DNA sequencing=== | ===DNA sequencing=== | ||
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:Genomic DNA >5ug (>200 ng/ul) | :Genomic DNA >5ug (>200 ng/ul) | ||
Revision as of 04:39, 13 November 2012
Contents |
Next generation sequencing
DNA sequencing
- Sample amounts
- Genomic DNA >5ug (>200 ng/ul)
RNAseq
- Sample RNA amounts
- Total RNA >10ug (concentration: >200 ng/ul)
- mRNA >1ug (concentration: >200 ng/ul)
- Sample RNA quality
- Intact rRNA should be observed in agarose gel electrophoresis total RNA preparation
- A260/280 ratio >1.8
- A260/230 ratio >1.8
