MiSeq
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==Useful links== | ==Useful links== | ||
;*[http://support.illumina.com/sequencing/sequencing_instruments/miseq/questions.ilmn| MiSeq Q&A] | ;*[http://support.illumina.com/sequencing/sequencing_instruments/miseq/questions.ilmn| MiSeq Q&A] | ||
| + | ;*[http://www.neb.com/nebecomm/tech_reference/NEBNext/NEBNext_illumina.asp#.UKMvq7ToSEk| NEBnext DNA library preparation kit] | ||
Revision as of 05:44, 14 November 2012
Contents |
Next generation sequencing
DNA sequencing
- Sample amounts
- Genomic DNA >5ug (>200 ng/ul)
RNAseq
- Sample RNA amounts
- Total RNA >10ug (concentration: >200 ng/ul)
- mRNA >1ug (concentration: >200 ng/ul)
- Sample RNA quality
- Intact rRNA should be observed in agarose gel electrophoresis total RNA preparation
- A260/280 ratio >1.8
- A260/230 ratio >1.8
