Molecular biology
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##[http://en.wikipedia.org/wiki/Phred_quality_score quality score] | ##[http://en.wikipedia.org/wiki/Phred_quality_score quality score] | ||
##good sequencing result vs. bad sequencing result (see below example) | ##good sequencing result vs. bad sequencing result (see below example) | ||
| - | [[image:sequencingresult.jpg|left|thumb|example]] | + | [[image:sequencingresult.jpg|left|thumb|good vs. bad sequencing example]] |
===protocols=== | ===protocols=== | ||
Revision as of 08:33, 21 July 2009
Contents |
Reagents & Protocols
recipes
- Chemicals
- Commonly used reagents and equipment in molecular biology (PDF)
- Concentration of antibiotics commonly used
- For DNA extraction (to remove some protein contaminants & inactivate enzymes): Phenol:Chloroform=1:1(v:v) solution
- DNA sequencing check list
- quality score
- good sequencing result vs. bad sequencing result (see below example)
protocols
- PCR - General rules for primer design
- Genomic DNA extraction
- Competent cell preparation BSGC protocol (PDF)
- Electrocompetent cell Protocol (PDF)
- LIC cloning introduction LIC introduction - Ligation Independent Cloning
- LIC cloning protocol (Berkeley Structural Genomics Center)
- Vector preparation
- Insert preparation
- LIC transformation:
- mix insert and vector in appropriate amounts
- incubate 5~30 min(20 min) at room temperature
- do transformation in E. coli (recommend to use electro-competent cells)
- LIC cloning protocol (Berkeley Structural Genomics Center)
- NCBE practical protocols: very good illustration for undergraduate students
- Conjugation
- Donor: E. coli S17-1
Tools
webtools
softwares
- NIH Image: Gel analysis software
- Serial Cloner: DNA sequence management (PCR, restriction maps, etc.)
- BioX (Mac OS X only)
- Biological sequence editor Bioedit
- Jmol
