Molecular biology

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Revision as of 03:14, 16 July 2010

Chemicals
Commonly used reagents and equipment in molecular biology (PDF)
Concentration of antibiotics commonly used
For DNA extraction (to remove some protein contaminants & inactivate enzymes): Phenol:Chloroform=1:1(v:v) solution
DNA sequencing check list
- quality score
- good sequencing result vs. bad sequencing result (see below example)

good vs. bad sequencing example

PCR - General rules for primer design
Genomic DNA extraction
Competent cell preparation BSGC protocol (PDF)
- Easy & rapid competent cell preparation reference link - by HJKo
Electrocompetent cell Protocol (PDF)
LIC cloning introduction LIC introduction - Ligation Independent Cloning
- LIC cloning protocol (Berkeley Structural Genomics Center)
  - Vector preparation
  - Insert preparation
  - LIC transformation:
    - mix insert and vector in appropriate amounts
    - incubate 5~30 min(20 min) at room temperature
    - do transformation in E. coli (recommend to use electro-competent cells)
NCBE practical protocols: very good illustration for undergraduate students
Conjugation
- Donor: E. coli S17-1
[[E. coli knockout]] by the Wanner method (Lambda Red Recombinase)

@@ Line 27: / Line 27: @@
 *Conjugation
 **Donor: <i>E. coli</i> [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=47055&Template=hosts S17-1]
+*[[<i>E. coli</i> knockout]] by the Wanner method (Lambda Red Recombinase)
 ==Tools==