Molecular biology
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==Reagents & Protocols== | ==Reagents & Protocols== | ||
===recipes=== | ===recipes=== | ||
Revision as of 13:10, 23 November 2010
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Reagents & Protocols
recipes
- Chemicals
- Commonly used reagents and equipment in molecular biology (PDF)
- Concentration of antibiotics commonly used
- For DNA extraction (to remove some protein contaminants & inactivate enzymes): Phenol:Chloroform=1:1(v:v) solution
- DNA sequencing check list
- quality score
- good sequencing result vs. bad sequencing result (see below example)
protocols
- PCR - General rules for primer design
- Genomic DNA extraction
- Competent cell preparation BSGC protocol (PDF)
- Easy & rapid competent cell preparation reference link - by HJKo
- Electrocompetent cell Protocol (PDF)
- LIC cloning introduction LIC introduction - Ligation Independent Cloning
Error fetching PMID 2235490:
- Error fetching PMID 2235490:
- LIC cloning protocol (Berkeley Structural Genomics Center)
- Vector preparation
- Insert preparation
- LIC transformation:
- mix insert and vector in appropriate amounts
- incubate 5~30 min(20 min) at room temperature
- do transformation in E. coli (recommend to use electro-competent cells)
- LIC cloning protocol (Berkeley Structural Genomics Center)
- NCBE practical protocols: very good illustration for undergraduate students
- Conjugation
- Donor: E. coli S17-1
- E. coli knockout by the Wanner method (Lambda Red Recombinase)
Tools
webtools
softwares
- NIH Image: Gel analysis software
- Serial Cloner: DNA sequence management (PCR, restriction maps, etc.)
- BioX (Mac OS X only)
- Biological sequence editor Bioedit
- Jmol
