Molecular biology

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		+	==Reagents & Protocols==
	*[[media:mba.pdf|Commonly used reagents and equipment in molecular biology (PDF)]]		*[[media:mba.pdf|Commonly used reagents and equipment in molecular biology (PDF)]]
	*[[PCR primer|PCR]] - General rules for primer design		*[[PCR primer|PCR]] - General rules for primer design

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Revision as of 02:55, 20 February 2008

Reagents & Protocols

• Commonly used reagents and equipment in molecular biology (PDF)
• PCR - General rules for primer design
• Genomic DNA extraction
• Competent cell preparation BSGC protocol (PDF)
• LIC cloning introduction LIC introduction - Ligation Independent Cloning
• LIC cloning protocol (Berkeley Structural Genomics Center)
  • Vector preparation
  • Insert preparation
  • LIC transformation
• Electrocompetent cell Protocol (PDF)
• Concentration of antibiotics commonly used

Softwares

• NIH Image: Gel analysis software
• Serial Cloner: DNA sequence management (PCR, restriction maps, etc.)
• Bioedit
• BioX (Mac OS X only)