Molecular biology

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	==Reagents & Protocols==		==Reagents & Protocols==
-	*[[media:mba.pdf|Commonly used reagents and equipment in molecular biology (PDF)]]	+	===reagents===
-	*[[PCR primer|PCR]] - General rules for primer design	+	#[[media:mba.pdf|Commonly used reagents and equipment in molecular biology (PDF)]]
-	*[[Genomic DNA extraction]]	+	#[[Concentration of antibiotics commonly used]]
-	*Competent cell preparation [[media:competentcell.pdf|BSGC protocol (PDF)]]	+	===protocols==
-	*LIC cloning introduction [[media:LICnovagen.pdf|LIC introduction]] - Ligation Independent Cloning	+	#[[PCR primer|PCR]] - General rules for primer design
-	*LIC cloning protocol (Berkeley Structural Genomics Center)	+	#[[Genomic DNA extraction]]
-	**[http://www.strgen.org/protocols/lic-vector-prep.pdf Vector preparation]	+	#Competent cell preparation [[media:competentcell.pdf|BSGC protocol (PDF)]]
-	**[http://www.strgen.org/protocols/lic-insert-prep.pdf Insert preparation]	+	#Electrocompetent cell [[media:ElectrocompetentEcoli.pdf|Protocol (PDF)]]
-	**[http://www.strgen.org/protocols/lic-rxn-transf.pdf LIC transformation]	+	#LIC cloning introduction [[media:LICnovagen.pdf|LIC introduction]] - Ligation Independent Cloning
-	*Electrocompetent cell [[media:ElectrocompetentEcoli.pdf|Protocol (PDF)]]	+	##LIC cloning protocol (Berkeley Structural Genomics Center)
-	*[[Concentration of antibiotics commonly used]]	+	###[http://www.strgen.org/protocols/lic-vector-prep.pdf Vector preparation]
		+	###[http://www.strgen.org/protocols/lic-insert-prep.pdf Insert preparation]
		+	###[http://www.strgen.org/protocols/lic-rxn-transf.pdf LIC transformation]
	==Softwares==		==Softwares==
-	*[[NIH Image]]: Gel analysis software	+	#[[NIH Image]]: Gel analysis software
-	*[http://serialbasics.free.fr/Serial_Cloner.html Serial Cloner]: DNA sequence management (PCR, restriction maps, etc.)	+	#[http://serialbasics.free.fr/Serial_Cloner.html Serial Cloner]: DNA sequence management (PCR, restriction maps, etc.)
-	*Bioedit	+	#Bioedit
-	*BioX (Mac OS X only)	+	#BioX (Mac OS X only)

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Revision as of 16:28, 5 July 2008

Contents 1 Reagents & Protocols 1.1 reagents 2 =protocols 3 Softwares

Reagents & Protocols

reagents

1. Commonly used reagents and equipment in molecular biology (PDF)
2. Concentration of antibiotics commonly used

=protocols

1. PCR - General rules for primer design
2. Genomic DNA extraction
3. Competent cell preparation BSGC protocol (PDF)
4. Electrocompetent cell Protocol (PDF)
5. LIC cloning introduction LIC introduction - Ligation Independent Cloning
   1. LIC cloning protocol (Berkeley Structural Genomics Center)
      1. Vector preparation
      2. Insert preparation
      3. LIC transformation

Softwares

1. NIH Image: Gel analysis software
2. Serial Cloner: DNA sequence management (PCR, restriction maps, etc.)
3. Bioedit
4. BioX (Mac OS X only)