Molecular biology

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	==Reagents & Protocols==		==Reagents & Protocols==
-	===reagents===	+	===recipes===
	#[[media:mba.pdf|Commonly used reagents and equipment in molecular biology (PDF)]]		#[[media:mba.pdf|Commonly used reagents and equipment in molecular biology (PDF)]]
	#[[Concentration of antibiotics commonly used]]		#[[Concentration of antibiotics commonly used]]
-	===protocols==	+	===protocols===
	#[[PCR primer|PCR]] - General rules for primer design		#[[PCR primer|PCR]] - General rules for primer design
	#[[Genomic DNA extraction]]		#[[Genomic DNA extraction]]
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	#Electrocompetent cell [[media:ElectrocompetentEcoli.pdf|Protocol (PDF)]]		#Electrocompetent cell [[media:ElectrocompetentEcoli.pdf|Protocol (PDF)]]
	#LIC cloning introduction [[media:LICnovagen.pdf|LIC introduction]] - Ligation Independent Cloning		#LIC cloning introduction [[media:LICnovagen.pdf|LIC introduction]] - Ligation Independent Cloning
-	##LIC cloning protocol (Berkeley Structural Genomics Center)	+	*LIC cloning protocol (Berkeley Structural Genomics Center)
-	###[http://www.strgen.org/protocols/lic-vector-prep.pdf Vector preparation]	+	#[http://www.strgen.org/protocols/lic-vector-prep.pdf Vector preparation]
-	###[http://www.strgen.org/protocols/lic-insert-prep.pdf Insert preparation]	+	#[http://www.strgen.org/protocols/lic-insert-prep.pdf Insert preparation]
-	###[http://www.strgen.org/protocols/lic-rxn-transf.pdf LIC transformation]	+	#[http://www.strgen.org/protocols/lic-rxn-transf.pdf LIC transformation]
	==Softwares==		==Softwares==
	#[[NIH Image]]: Gel analysis software		#[[NIH Image]]: Gel analysis software

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Revision as of 16:30, 5 July 2008

Contents 1 Reagents & Protocols 1.1 recipes 1.2 protocols 2 Softwares

Reagents & Protocols

recipes

1. Commonly used reagents and equipment in molecular biology (PDF)
2. Concentration of antibiotics commonly used

protocols

1. PCR - General rules for primer design
2. Genomic DNA extraction
3. Competent cell preparation BSGC protocol (PDF)
4. Electrocompetent cell Protocol (PDF)
5. LIC cloning introduction LIC introduction - Ligation Independent Cloning

• LIC cloning protocol (Berkeley Structural Genomics Center)

1. Vector preparation
2. Insert preparation
3. LIC transformation

Softwares

1. NIH Image: Gel analysis software
2. Serial Cloner: DNA sequence management (PCR, restriction maps, etc.)
3. Bioedit
4. BioX (Mac OS X only)