Molecular biology
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#[http://www.strgen.org/protocols/lic-vector-prep.pdf Vector preparation] | #[http://www.strgen.org/protocols/lic-vector-prep.pdf Vector preparation] | ||
#[http://www.strgen.org/protocols/lic-insert-prep.pdf Insert preparation] | #[http://www.strgen.org/protocols/lic-insert-prep.pdf Insert preparation] | ||
- | #[http://www.strgen.org/protocols/lic-rxn-transf.pdf LIC transformation] | + | #[http://www.strgen.org/protocols/lic-rxn-transf.pdf LIC transformation]: |
+ | ##mix insert and vector in appropriate amounts | ||
+ | ##incubate 5~30 min(20 min) at room temperature | ||
+ | ##do transformation in E. coli (recommend to use electro-competent cells) | ||
+ | |||
==Softwares== | ==Softwares== | ||
#[[NIH Image]]: Gel analysis software | #[[NIH Image]]: Gel analysis software |
Revision as of 06:43, 9 July 2008
Contents |
Reagents & Protocols
recipes
- Commonly used reagents and equipment in molecular biology (PDF)
- Concentration of antibiotics commonly used
protocols
- PCR - General rules for primer design
- Genomic DNA extraction
- Competent cell preparation BSGC protocol (PDF)
- Electrocompetent cell Protocol (PDF)
- LIC cloning introduction LIC introduction - Ligation Independent Cloning
- LIC cloning protocol (Berkeley Structural Genomics Center)
- Vector preparation
- Insert preparation
- LIC transformation:
- mix insert and vector in appropriate amounts
- incubate 5~30 min(20 min) at room temperature
- do transformation in E. coli (recommend to use electro-competent cells)
Softwares
- NIH Image: Gel analysis software
- Serial Cloner: DNA sequence management (PCR, restriction maps, etc.)
- Bioedit
- BioX (Mac OS X only)