Molecular biology

From CSBLwiki

Reagents & Protocols

• Commonly used reagents and equipment in molecular biology (PDF)
• PCR - General rules for primer design
• Genomic DNA extraction
• Competent cell preparation BSGC protocol (PDF)
• LIC cloning introduction LIC introduction - Ligation Independent Cloning
• LIC cloning protocol (Berkeley Structural Genomics Center)
  • Vector preparation
  • Insert preparation
  • LIC transformation
• Electrocompetent cell Protocol (PDF)
• Concentration of antibiotics commonly used

Softwares

• NIH Image: Gel analysis software
• Serial Cloner: DNA sequence management (PCR, restriction maps, etc.)
• Bioedit
• BioX (Mac OS X only)