Molecular biology

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Contents 1 Reagents & Protocols 1.1 reagents 2 =protocols 3 Softwares

Reagents & Protocols

reagents

1. Commonly used reagents and equipment in molecular biology (PDF)
2. Concentration of antibiotics commonly used

=protocols

1. PCR - General rules for primer design
2. Genomic DNA extraction
3. Competent cell preparation BSGC protocol (PDF)
4. Electrocompetent cell Protocol (PDF)
5. LIC cloning introduction LIC introduction - Ligation Independent Cloning
   1. LIC cloning protocol (Berkeley Structural Genomics Center)
      1. Vector preparation
      2. Insert preparation
      3. LIC transformation

Softwares

1. NIH Image: Gel analysis software
2. Serial Cloner: DNA sequence management (PCR, restriction maps, etc.)
3. Bioedit
4. BioX (Mac OS X only)