Newbler
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| - | Tips for Newbler <i>de novo</i> assembler | + | =Tips for Newbler <i>de novo</i> assembler= |
*454Scaffolds: Contigs with gaps or contigs longer than 2000bp | *454Scaffolds: Contigs with gaps or contigs longer than 2000bp | ||
*454AllContigs: If there are multi copy contigs, the Newbler make them island. | *454AllContigs: If there are multi copy contigs, the Newbler make them island. | ||
Revision as of 01:58, 3 November 2010
Tips for Newbler de novo assembler
- 454Scaffolds: Contigs with gaps or contigs longer than 2000bp
- 454AllContigs: If there are multi copy contigs, the Newbler make them island.
- gapResolution: can cover this problem by local assembly.
- We can manually check with 454PairStatus.txt file.
- It is important to know which contig is multicopy and how many it(orgarnism) has.
