PCR primer

From CSBLwiki

Revision as of 17:20, 5 January 2008 (view source) Csblsysop (Talk | contribs) ← Older edit		Revision as of 17:26, 5 January 2008 (view source) Csblsysop (Talk | contribs) Newer edit →
Line 2:		Line 2:
	**Have primers at 100uM concentration and diluted to 0.1~0.5uM for the reaction mixture.		**Have primers at 100uM concentration and diluted to 0.1~0.5uM for the reaction mixture.
	*Qiagen recommendation [[Media:pcrcha05.pdf|PDF]]		*Qiagen recommendation [[Media:pcrcha05.pdf|PDF]]
		+	*Online-protocols [http://www.protocol-online.org/prot/Molecular_Biology/PCR/ PCR]

---

Revision as of 17:26, 5 January 2008

• PCR primer concentration
  • Have primers at 100uM concentration and diluted to 0.1~0.5uM for the reaction mixture.
• Qiagen recommendation PDF
• Online-protocols PCR