PCR primer

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	*PCR primer concentration		*PCR primer concentration
	**Have primers at 100uM concentration and diluted to 0.1~0.5uM for the reaction mixture.		**Have primers at 100uM concentration and diluted to 0.1~0.5uM for the reaction mixture.
		+	*dNTP - 0.2mM, MgCl<sub>2</sub> - 1~4mM
	*Qiagen recommendation [[Media:pcrcha05.pdf|PDF]]		*Qiagen recommendation [[Media:pcrcha05.pdf|PDF]]
	*Online-protocols [http://www.protocol-online.org/prot/Molecular_Biology/PCR/ PCR]		*Online-protocols [http://www.protocol-online.org/prot/Molecular_Biology/PCR/ PCR]

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Revision as of 17:33, 5 January 2008

• PCR primer concentration
  • Have primers at 100uM concentration and diluted to 0.1~0.5uM for the reaction mixture.
• dNTP - 0.2mM, MgCl₂ - 1~4mM
• Qiagen recommendation PDF
• Online-protocols PCR