RNAseq

From CSBLwiki

External links

RNA-seq analysis HowTo

Bioconductor

Using Bioconductor for Sequence Data

Informative Q&A for bacterial RNA-seq processing (GFF file for HTseq-count)

GFF format GFF is a format for locating & describing genes and other localized features associated with DNA, RNA and Protein sequences.

12/20/2011 RPKM (reads per kilobase per million reads) = 10^9 * C / (N * L), where C = # of mappable reads to a gene (exons), N = total # of mappable reads (size of library), and L = size of gene (bp) or sum of eons http://www.ncbi.nlm.nih.gov/pubmed/18516045?dopt=Abstract