From mycopedia
(Created page with "= PAHs tolerance = All white rot fungi were tested by culture on 2% MEA supplemented with 30 ppm each of anthracene, phenanthrene, fluorancthene and pyrene. The PAHs (Sigma Aldr...") |
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| + | = Pyrene = | ||
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= PAHs tolerance = | = PAHs tolerance = | ||
| − | All white rot fungi were tested by culture on 2% MEA supplemented with 30 ppm each of anthracene, phenanthrene, fluorancthene and pyrene. The PAHs (Sigma Aldrich, St. Louis, USA) were of analytical grade. They are almost completely insoluble in water; therefore, acetone (>99 %; Junsei Chemical, Japan) was used to dissolve them in the MEA medium. One L of MEA was autoclaved and then cooled to ≈.45 °C, after which the PAHs were added in 7 mL acetone solution. The agar plates were stored at room temperature for 7 d on a clean bench to allow the acetone to evaporate prior to inoculation with the fungus. White rot fungi were then inoculated onto Petri dishes spiked with each PAH and incubated at 27°C. After incubation, the tolerant growth rate TGR = FGR/GRC × 100 (where GRC is growth rate of control culture and FGRs fungal growth rate) was determined by measuring the diameter of the expanding colonies. The tolerance rate was expressed as +++++ (100 ≥ % TGR > 90), ++++ (90 ≥ % TGR > 70), +++ (70 ≥ % TGR > 50), ++ (50 ≥ % TGR > 30), + (30 ≥ % TGR > 0), and ☆☆☆☆☆ (no tolerance). | + | All white rot fungi were tested by culture on 2% MEA supplemented with 30 ppm each of anthracene, phenanthrene, fluorancthene and pyrene. The PAHs (Sigma Aldrich, St. Louis, USA) were of analytical grade. They are almost completely insoluble in water; therefore, acetone (>99 %; Junsei Chemical, Japan) was used to dissolve them in the MEA medium. One L of MEA was autoclaved and then cooled to ≈.45 °C, after which the PAHs were added in 7 mL acetone solution. The agar plates were stored at room temperature for 7 d on a clean bench to allow the acetone to evaporate prior to inoculation with the fungus. White rot fungi were then inoculated onto Petri dishes spiked with each PAH and incubated at 27°C. After incubation, the tolerant growth rate TGR = FGR/GRC × 100 (where GRC is growth rate of control culture and FGRs fungal growth rate) was determined by measuring the diameter of the expanding colonies. |
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| + | The tolerance rate was expressed as +++++ (100 ≥ % TGR > 90), ++++ (90 ≥ % TGR > 70), +++ (70 ≥ % TGR > 50), ++ (50 ≥ % TGR > 30), + (30 ≥ % TGR > 0), and ☆☆☆☆☆ (no tolerance). | ||
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=reference = | =reference = | ||
Lee et al. 2010. Folia Microbiol. 55: 447-453 | Lee et al. 2010. Folia Microbiol. 55: 447-453 | ||
Latest revision as of 16:19, 24 April 2013
[edit] Pyrene
[edit] PAHs tolerance
All white rot fungi were tested by culture on 2% MEA supplemented with 30 ppm each of anthracene, phenanthrene, fluorancthene and pyrene. The PAHs (Sigma Aldrich, St. Louis, USA) were of analytical grade. They are almost completely insoluble in water; therefore, acetone (>99 %; Junsei Chemical, Japan) was used to dissolve them in the MEA medium. One L of MEA was autoclaved and then cooled to ≈.45 °C, after which the PAHs were added in 7 mL acetone solution. The agar plates were stored at room temperature for 7 d on a clean bench to allow the acetone to evaporate prior to inoculation with the fungus. White rot fungi were then inoculated onto Petri dishes spiked with each PAH and incubated at 27°C. After incubation, the tolerant growth rate TGR = FGR/GRC × 100 (where GRC is growth rate of control culture and FGRs fungal growth rate) was determined by measuring the diameter of the expanding colonies.
The tolerance rate was expressed as +++++ (100 ≥ % TGR > 90), ++++ (90 ≥ % TGR > 70), +++ (70 ≥ % TGR > 50), ++ (50 ≥ % TGR > 30), + (30 ≥ % TGR > 0), and ☆☆☆☆☆ (no tolerance).
[edit] reference
Lee et al. 2010. Folia Microbiol. 55: 447-453