14.4.11
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1.pSB1C3 다시 접종
2. 10일에 gel prep 한 srt A gene cluster 를 template로
PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol
PCR for pEKEx2-srtA gene cluster cloning into pSB1C3 with Q5 pol
<조성>
rxn buffer 10
dNTP 1
F.primer 2.5
R.primer 2.5
Template 1
Q5 pol 0.5
Q5 GC 10
DW 22.5
up to 50
<condition>
98 30
-98 10
68 15'- *30
72 10' 16 --
실패