14.4.16

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PCR srt A gene cluster for gibson assembly with Q5 pol PCR pEKEx2 for gibson assembly with Q5 pol

template : genomic DNA, pEKEx2

<조성>

rxn buffer 10

dNTP 1

F.primer 2.5

R.primer 2.5

Template 2

Q5 pol 0.5

Q5 GC 10

DW 21.5

up to 50

<condition-srtA>

98 30

-98 10'

55,60,65 30

72 5'-*35

72 10'

16--

실패

<condition-pEKEx2>

98 30

-98 10'

68 15'-*35

72 10'

16 --

성공-gel prep