8.26

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pATLIC : 단백질 표면발현벡터

PATLIC.png

근데 Nacrein은 원래 signal peptide가 달려있는 놈이라 이걸 떼고 클로닝햇어야했느데 안떼고해서 다시 클로닝

plasmid prep

pATLIC prep

PCR

Nacrein pcr


temp 1

10xbuffer 5

dNTP 2.5

primer 2

HQ 5

a-Taq 0.5

DW 34

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