Alpha-Taq pol

Jump to: navigation, search

Reaction mixture

10x alpha-Taq reaction buffer 5 uL

dNTP mix(2.5mM) 3 uL

primer 2 uL

templete-genomic DNA 3 uL

alpha-Taq(2.5U/ul) 0.5ul

DW up to 50ul

(보통 extension 0.5-1min/kb)

95'C 2min

95'C 20sec

55'C 2min

72'C 2min

72'C 5min

alpha-Taq은 효율이 좋은 pol이기 때문에 0.5-1 ul만 넣고 전기영동해도 충분