Gene replacement
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E. coli Gene Replacement
pKO3 & pKOV system
- sacB
- pSC101-ts (30C, 43C), cat
- natural recombination
- knockout - looking for integrates (30C->43C)
- marker free - sacB (50:50)
pKNOCK system
- R6k-gamma (pir+ dependent)
- RP4 (oriT)
- natural recombination
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