14.4.10

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	pSB1C3 다시 접종		pSB1C3 다시 접종
		+	PCR for pSB1c3-srtA gene cluster cloning with Q5 pol
	PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol		PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol
		+
		+	<조성>
		+
		+	Q5 Rxn buffer 10
		+
		+	dNTP 1
		+
		+	F,R primer 2.5
		+
		+	Template 2 ->srtA gene cluster, pEKEx2
		+
		+	Q5 pol 0.5
		+
		+	Q5 GC 10
		+
		+	DW 21.5
		+
		+	up to 50
		+
		+
		+	<PCR condition>
		+
		+	3-step, Touchdown 조건 안적어놔서 모름
		+
		+	3-step으로 "PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol" pEKEx2랑 srtA gene cluser 성공 -> srtA gene cluster gel prep
		+
		+	Touchdown으로 "PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol" pEKEx2성공

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Latest revision as of 07:21, 20 July 2014

pSB1C3 다시 접종

PCR for pSB1c3-srtA gene cluster cloning with Q5 pol PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol

<조성>

Q5 Rxn buffer 10

dNTP 1

F,R primer 2.5

Template 2 ->srtA gene cluster, pEKEx2

Q5 pol 0.5

Q5 GC 10

DW 21.5

up to 50

<PCR condition>

3-step, Touchdown 조건 안적어놔서 모름

3-step으로 "PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol" pEKEx2랑 srtA gene cluser 성공 -> srtA gene cluster gel prep

Touchdown으로 "PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol" pEKEx2성공