User contributions
From CSBLwiki
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- 07:51, 20 July 2014 (diff | hist) N 14.5.2 (Created page with "Oneshot-gibson product : electroporation") (top)
- 07:50, 20 July 2014 (diff | hist) N 14.5.1 (Created page with "2ml 접종한것 nutrient broth 100ml에 배양 -안자라..") (top)
- 07:50, 20 July 2014 (diff | hist) N 14.4.29 (Created page with "''Corynebacterium glutamicum'' comp cell 만들기 -2 ml LB 에 접종") (top)
- 07:49, 20 July 2014 (diff | hist) N 14.4.28 (Created page with "25일에 gibson transformation한거 접종") (top)
- 07:48, 20 July 2014 (diff | hist) N 14.4.25 (Created page with "gibson assembly 다시 (2* master mix) 후 transformation : 콜로리 떳다. pCLTon2 tranformation -안되...엉엉") (top)
- 07:46, 20 July 2014 (diff | hist) N 14.4.24 (Created page with "1. streptomycin LB plate 만들기 2. gibson assembly(2* master mix) experiment : pilin 9 : pEKEx2 1 + mix 10 control(positive) : 5 + mix 5 assembly 된거 바로 ''E.coli'' ...") (top)
- 07:43, 20 July 2014 (diff | hist) Undergraduate 유민섭 (top)
- 07:42, 20 July 2014 (diff | hist) 14.4.16 (top)
- 07:42, 20 July 2014 (diff | hist) 14.4.16
- 07:42, 20 July 2014 (diff | hist) 14.4.16
- 07:41, 20 July 2014 (diff | hist) N 14.4.16 (Created page with "PCR srt A gene cluster for gibson assembly with Q5 pol PCR pEKEx2 for gibson assembly with Q5 pol template : genomic DNA, pEKEx2 <조성> rxn buffer 10 dNTP 1 F.primer 2.5 ...")
- 07:37, 20 July 2014 (diff | hist) 14.4.15 (top)
- 07:34, 20 July 2014 (diff | hist) 14.4.14 (top)
- 07:34, 20 July 2014 (diff | hist) N 14.4.15 (Created page with "PCR srt A gene cluser for gibson assembly and cloning into pSB1C3 with Q5 pol template <조성> rxn buffer 10 dNTP 1 F.primer 2.5 R.primer 2.5 Template 2 Q5 pol 0.5 Q...")
- 07:32, 20 July 2014 (diff | hist) N 14.4.14 (Created page with "PCR srt A gene cluster for gibson assembly with Q5 pol <조성> rxn buffer 10 dNTP 1 F.primer 2.5 R.primer 2.5 Template 2 Q5 pol 0.5 Q5 GC 10 DW 22.5 up to 50 <conditi...")
- 07:30, 20 July 2014 (diff | hist) 14.4.11 (top)
- 07:29, 20 July 2014 (diff | hist) N 14.4.11 (Created page with "1.pSB1C3 다시 접종 2. 10일에 gel prep 한 srt A gene cluster 를 template로 PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol PCR for pEKEx2-srtA gene cl...")
- 07:21, 20 July 2014 (diff | hist) 14.4.10 (top)
- 13:09, 24 June 2014 (diff | hist) N 14.4.12 (Created page with "pSB1C3 추출") (top)
- 07:17, 24 June 2014 (diff | hist) 14.4.10
- 08:40, 23 June 2014 (diff | hist) N 14.4.10 (Created page with "pSB1C3 다시 접종 PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol")
- 08:34, 23 June 2014 (diff | hist) N 14.4.8 (Created page with "보다 증폭잘된다는 TAKARA LA Taq 사용 1. srtA gene cluster for pSB1C3 cloning PCR with LA Taq <조성> LA Taq 0.5 Rxn buffer 5 MgCl2 5 dNTP 8 F,R Primer 4 Templa...") (top)
- 08:29, 23 June 2014 (diff | hist) N 14.4.7 (Created page with "srtA gene cluster for pSB1C3 cloning PCR with Q5 pol <조성> Q5 rxn buffer : 10 dNTP : 1 F.R primer : 2.5 Template : 2 Q5 pol : 0.5 Q5 GC : 10 DW : 21.5 up to 50 < P...") (top)
- 08:24, 23 June 2014 (diff | hist) 14.4.2 (top)
- 08:24, 23 June 2014 (diff | hist) N 14.4.2 (Created page with "pSB1c3-srtA gene cluster cloning 할 때 쓸 srtA gene cluster PCR primer 주문")
- 08:22, 23 June 2014 (diff | hist) N 14.4.1 (Created page with "srtA gene cluster 확인") (top)
- 08:22, 23 June 2014 (diff | hist) N 14.3.31 (Created page with "pSB1C3 , pEKEx2 size check") (top)
- 08:21, 23 June 2014 (diff | hist) N 14.3.29 (Created page with "pEKEx2 plasmid 추출") (top)
- 08:21, 23 June 2014 (diff | hist) 14.3.28 (top)
- 08:20, 23 June 2014 (diff | hist) 14.3.26 (top)
- 08:19, 23 June 2014 (diff | hist) N 14.3.28 (Created page with "Oneshot-pEKEx2 : LB 3ml 접종 Oneshot-pCLTon2 : transformation Agar")
- 08:18, 23 June 2014 (diff | hist) N 14.3.27 (Created page with "pSB1C3 plasmid 추출 Oneshot-pEKEx2 : transformation Spectinomycin 배지 만들기") (top)
- 08:17, 23 June 2014 (diff | hist) N 14.3.26 (Created page with "Oneshot-pSB1C3 : LB 3ml 접종 Uniprot에서 Agarase pili에 적합한 agarase 알아보기 N,C terminal 쪽에 catabolytic domain 없이 잘 빠져나와있고, monomer에 b-...")
- 08:13, 23 June 2014 (diff | hist) N 14.3.25 (Created page with "pSB1C3 증폭위해 Oneshot-pSB1C3 transformation") (top)
- 08:09, 23 June 2014 (diff | hist) Undergraduate 유민섭
- 12:16, 26 March 2014 (diff | hist) N Agarse screening (Created page with "http://www.uniprot.org/uniprot/?query=job%3a2014032662AJZWU6QE&format=tab&columns=id,entry%20name,reviewed,protein%20names,genes,organism,length") (top)
- 12:16, 26 March 2014 (diff | hist) Undergraduate 유민섭
- 04:18, 17 March 2014 (diff | hist) N Taq 정제방법 (Created page with "sonification 해서 원심분리 후 soup만 75도에서 1시간 가열하면 Taq말고 다른 대장균단백질은 다 aggrigation되서 가라앉는다 가열 후 soup만 c...") (top)
- 04:17, 17 March 2014 (diff | hist) Undergraduate 유민섭
- 04:16, 17 March 2014 (diff | hist) N 14.3.13 (Created page with "호구 GFP 희석않고 reaction test 했더니 다 끌림...") (top)
- 04:15, 17 March 2014 (diff | hist) N 14.3.12 (Created page with "11일에 배양한 GFP 정제") (top)
- 04:15, 17 March 2014 (diff | hist) N 14.3.11 (Created page with "10일에 접종한거 AIM에 배양 호구 GFP로 reaction test 했으나 GFP가 생각보다 농도가 옅다??") (top)
- 04:14, 17 March 2014 (diff | hist) N 14.3.10 (Created page with "Bl21-2*Ala:GFP만 다시 접종") (top)
- 04:13, 17 March 2014 (diff | hist) N 14.3.6 (Created page with "GFP 10배 희석, mRFP 30배 희석해서 PAGE걸었는데 gfp가 이상하다") (top)
- 04:13, 17 March 2014 (diff | hist) N 14.3.5 (Created page with "4일에 농축한것 정량 srtA-2.09 mg/ml GFP-25.1 mRFP-63.5-74.4") (top)
- 04:12, 17 March 2014 (diff | hist) N 14.3.4 (Created page with "새로 정제한 srtA, GFP,mRFP 농축") (top)
- 04:12, 17 March 2014 (diff | hist) N 14.2.26 (Created page with "25일에 induction한거 cell harvest, sonification, centrifuge 후 soup만 75도에서 1시간 가열, 원심분리 후 soup만 centricon으로 농축 - Taq 정제 25일에 ...") (top)
- 04:10, 17 March 2014 (diff | hist) N 14.2.25 (Created page with "24일에 접종한거 1L large scale up 후 induction streaking한거 접종") (top)
- 04:09, 17 March 2014 (diff | hist) N 14.2.24 (Created page with "Taq Pol DIY로 만들기 프로젝트 Taq 클로닝 된거 plate에 streaking lb에 접종") (top)
- 04:08, 17 March 2014 (diff | hist) N 14.2.21 (Created page with "srtA,mRFP,GFP 다시 정제") (top)
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