14.4.14
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(Created page with "PCR srt A gene cluster for gibson assembly with Q5 pol <조성> rxn buffer 10 dNTP 1 F.primer 2.5 R.primer 2.5 Template 2 Q5 pol 0.5 Q5 GC 10 DW 22.5 up to 50 <conditi...") |
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Latest revision as of 07:34, 20 July 2014
PCR srt A gene cluster for gibson assembly with Q5 pol
template : genomic DNA
<조성>
rxn buffer 10
dNTP 1
F.primer 2.5
R.primer 2.5
Template 2
Q5 pol 0.5
Q5 GC 10
DW 22.5
up to 50
<condition>
98 30
-98 10
68 15'-*30
72 10'
16 --
실패
