14.4.15

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Revision as of 07:34, 20 July 2014 (view source) Guest (Talk | contribs) (Created page with "PCR srt A gene cluser for gibson assembly and cloning into pSB1C3 with Q5 pol template <조성> rxn buffer 10 dNTP 1 F.primer 2.5 R.primer 2.5 Template 2 Q5 pol 0.5 Q...") ← Older edit		Latest revision as of 07:37, 20 July 2014 (view source) Guest (Talk | contribs)
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	PCR srt A gene cluser for gibson assembly and cloning into pSB1C3 with Q5 pol		PCR srt A gene cluser for gibson assembly and cloning into pSB1C3 with Q5 pol
-	template	+	template - genomic DNA and 4/11에 prep 한 product
-		+
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	up to 50		up to 50
		+
		+	<condition>
		+
		+	98 30''
		+
		+	-98 10''
		+
		+	60 30''
		+
		+	72 5'-*35
		+
		+	72 10'
		+
		+	16 --
		+
		+	실패

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Latest revision as of 07:37, 20 July 2014

PCR srt A gene cluser for gibson assembly and cloning into pSB1C3 with Q5 pol

template - genomic DNA and 4/11에 prep 한 product

<조성>

rxn buffer 10

dNTP 1

F.primer 2.5

R.primer 2.5

Template 2

Q5 pol 0.5

Q5 GC 10

DW 22.5

up to 50

<condition>

98 30

-98 10

60 30

72 5'-*35

72 10'

16 --

실패