14.4.15

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PCR srt A gene cluser for gibson assembly and cloning into pSB1C3 with Q5 pol

template - genomic DNA and 4/11에 prep 한 product

<조성>

rxn buffer 10

dNTP 1

F.primer 2.5

R.primer 2.5

Template 2

Q5 pol 0.5

Q5 GC 10

DW 22.5

up to 50

<condition>

98 30

-98 10

60 30

72 5'-*35

72 10'

16 --

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